FluoroDish™ dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection, and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish™ uses a specially formulated adhesive that is optically clear and durable with extremely low toxicity. The bottom glass has superior UV transmission: 30% transmission at 300 nm, compared to less than 7% for the most popular German glass. Stringent quality control ensures that glass thickness stays within the 0.17 ±0.01 mm range. Cited in 575 reference articles (in the NIH PubMed Central® alone), our optical grade, glass bottom culture dishes are unique in the marketplace and conform to strict quality control standards.

Conventional plastic dishes and chambers limit the utility of the inverted scope for many applications, as thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17mm thick) optical quality glass bottom, allowing the use of a much shorter objective working distance, larger numerical aperture (NA), and a higher magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with a medium such as water, glycerin, or oil for the highest magnification possible. To optimize heat exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

WPI offers a range of coated 35mm dishes, including Collagen, Fibronectin, Poly-D-Lysine, Poly-L-Lysine, and Vitronectin. Coated culture dishes are useful in promoting cell adhesion to culture surface and can be used for cell attachment, cell expansion, serum-free cultures, and cancer research. Additionally, coated dishes allow for detailed observation of cellular process when doing high-resolution live cell imaging analysis. When doing cell adhesion assays, coated dishes facilitate studying cell-substrate interations. Coated culture dishes are suitable for microinjection experiments, too.

WPI’s Blackwall FluoroDishes™ have low background fluorescence, making them ideal for live cell microscopy, as well as fluorescence microscopy, other high-resolution microscopy techniques, confocal microscopy, and inverted microscopy. They are also a good choice for applications like Total Internal Reflection Fluorescence (TIRF) and super-resolution microscopy. When working in a dark room, the black dishes allow for better visualization of the meniscus of the media. Blackwall FluoroDishes™ have a low rim and sidewall, making them suitable for micromanipulation or microinjection experiments.

• Optical quality glass bottom for shorter working distances, larger numerical aperture and higher magnification
• Non-fluorescent glass so you can discern weaker signals
• Allows the use of immersion objectives
• Flat bottom optimizes heat exchange
• Less optical distortion and superior UV transmission
• Low cytotoxicity adhesive to ensure cells' survival
• Individually packaged and gamma sterilized
• Coated dishes available for better cell adhesion
• Blackwall dishes are available to block stray lights and reduce background noise