EVOM™ Electrode for TEER

For TEER measurement of epithelial and endothelial cell cultures

• The new EndOhm chamber upper mount is made of polycarbonate and unaffected by alcohol
• The glass chamber is easier to clean and more scratch resistant than the prior versions. The EndOhm is not recommended for incubator use due to risk of the glass cracking.
• Adjustable apical electrode height
• Crystal clear glass chamber allows visualization of apical electrode positioning
• New insert holder with 120º tri-supports for three leg inserts
• Three sizes cover a range of well cup sizes from a variety of manufacturers
• Compatible with EVOM3 and EVOM™ Manual

To find the equivalent EndOhm chambers compatible with EVOM, EVOM2™, and Millicell^® ERS and ERS2, visit the product page here.

Options

Benefits

• Stability and reproducibility superior to the STX4 electrodes to 1% tolerance
• Can be used with 6, 12 or 24 well plates with removable inserts
• Symmetrical electrode pattern disperses test current uniformly
• Tri-leg supports offer mechanical stability and the membrane is held parallel to the electrodes
• Simple test procedure to verify electrode performance
• Premium Warranty Available

Applications

• TEER measurement for removable culture cup systems using EVOM™ Manual meters for endothelial and epithelial cell cultures

Make more precise measurements with EndOhms

EndOhm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with the basic STX4 electrodes. The background resistance of a blank insert is reduced from 150 Ω (when using WPI’s hand-held STX4 electrodes) to less than 5 Ω. With EndOhm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX4 electrodes (depending on the experience of the user) to 1-2 Ω. Compared with other resistance measurement methods, EndOhm with an EVOM meter offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM™ Manual, errors owing to electrode polarization or membrane capacitance are largely eliminated. EndOhm together with EVOM™ Manual offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Corning, Millipore, Nunc, Greiner and BD Falcon have been tested. EndOhm chambers may be sterilized with EtO, alcohol or a bactericide; not autoclavable.

NOTE: EndOhm chambers have Ag/AgCl electrodes. If you are planning to do continuous data recording for extended period, you may need to take into consideration any potential cytotoxic effects of long-term exposure of silver on your specific cell type.

Compatibility Charts

The ENDOHM-6 (#EVM-EL-03-01-01) is compatible with the following chambers:

* The tri-supports overhang the chamber edge and the well cannot be held parallel to the electrodes.

The ENDOHM-12 (#EVM-EL-03-01-02) is compatible with the following chambers:

* The tri-supports legs must be balance correctly so that the filter is parallel to the electrodes.

The ENDOHM-24 (#EVM-EL-03-01-03) is compatible with the following chambers:

* The tri-support legs must be balanced correctly so that the filter is parallel to the electrodes.

Frequently Asked Questions