Gas-Tight Microinjection System

The NanoFil™ Gas-Tight Injection System boasts zero dead volume, an the variability is eliminated with the NanoFil™ design: our interchangable needles advance directly into the syringe barrel, creating a 1:1 connection with the plunger— a truly gas-tight system built for low-volume sample control. Our cross-compatible NanoFil™ needles are offered in sizes from 33-36g, blunt or beveled.
* Returns Policy : Non-returnable/Non-refundable
Curious how NanoFilâ„¢ supports precision microinjection?
Visit the Gas-Tight Injection System page to learn about its gas-tight design, needle versatility, etc.
Options
| Order code | Size | Includes |
| NANOFIL | 10 μL |
|
| NANOFIL-100 | 100 µL |
|
Features & Applications
- Biologically compatible injection syringe
- Compatible with WPI's UMP3 microinjector for precise, targeted injections
- Animal research, viral targeting (e.g. AAV construct, DREADDS):
- Retinal Pigment Epithelial (RPE)/ Intra Ocular (IO)
- Brain
- Muscle
- Spine
- Capillary electrophoresis
Try our quartz 34G flexible tubing for precision capillary backfilling (SKU #NFQ34-5). Particularly useful for low-volume sample transfer into long taper glass pipettes. Compatible with any NanoFilTM syringe!




Special Application Kits

More Information
How to select the correct tip for your application.
See why our system is the choice for low-volume applications, and why other designs are not dependable: Tired of Missing the Mark?
Note: This product is non-returnable/non-refundable. For details, please refer to the WPI Terms & Conditions.
*After repeated use, it is normal that your plunger may incur wear/tear to either the PTFE tip and/or its overall straightness. The syringe must be replaced as plungers are specially made to each syringe.
References
Guo, Q., Gobbo, D., Zhao, N., Zhang, H., Awuku, N.O., Liu, Q., Fang, L.P., Gampfer, T.M., Meyer, M.R., Zhao, R., Bai, X., Bian, S., Scheller, A., Kirchhoff, F., & Huang, W. (2024). Adenosine triggers early astrocyte reactivity that provokes microglial responses and drives the pathogenesis of sepsis-associated encephalopathy in mice. Nature Communications, 15; 6340. https://doi.org/10.1038/s41467-024-50466-y
Luo, S., Jiang, H., Li, Q., Qin, Y., Yang, S., Li, J., Xu, L., Gou, Y., Zhang, Y., Liu, F., Ke, X., Zheng, Q., & Sun, X. (2024). An adeno-associated virus variant enabling efficient ocular-directed gene delivery across species. Nature communications, 15(1), 3780. https://doi.org/10.1038/s41467-024-48221-4
Nam, J., Richie, C.T., Harvey, B.K., & Voutilainen, M.H. (2024). Delivery of CDNF byAAV‑mediated gene transfer protects dopamine neurons and regulates ER stress and inflammation in an acute MPTP mouse model of Parkinson’s disease. Communications Biology, 7; 966. https://doi.org/10.1038/s42003-024-06658-9
Wietek, J., Nozownik, A., Pulin, M., Saraf-Sinik, I., Matosevich, N., Gowrishankar, R., Gat, A., Malan, D., Brown, B.J., Dine, J., Imambocus, B.N., Levy, R., Sauter, K., Litvin, A., Regev, N., Subramaniam, S., Abrera, K., Summarli, D., Goren, E.M., Mizrachi, G., Bitton, E., Benjamin, A., Copits, B.A., Sasse, P., Rost, B.R., Schmitz, D., Bruchas, M.R., Soba, P., Oren-Suissa, M., Nir, Y., Wiegert, J.S., & Yizhar, O. (2024). A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits. Nature Methods, 21; pg. 1275–1287. https://doi.org/10.1038/s41592-024-02285-8
Zhang, C., Dulinskas, R., Ineichen, C., Greter, A., Sigrist, H., Li, Y., Alanis-Lobato, G., Hengerer, B., & Pryce, C.R. (2024). Chronic stress deficits in reward behaviour co-occur with low nucleus accumbens dopamine activity during reward anticipation specifically. Communications Biology, 7; 966. https://doi.org/10.1038/s42003-024-06658-9
| SKU | VAR-3167 |
|---|
- As low as $53.00
- As low as $761.00
- Request Quote
Documents
NanoFilâ„¢ Gas-Tight Injection System Manual
NanoFilâ„¢ Gas-Tight Injection System Spec Sheet
Selecting Your NanoFilâ„¢ Needle Tip
Video
How to Prime Your NanoFil Gas-Tight Syringe System
Unboxing Your NanoFil & Installing a Needle
In the video below, you can see how to front fill an Nanofil syringe.
How to Choose the Correct NanoFil Tip Size for Microinjections
How to Use the NanoFil Syringe for Retinal Pigment Epithelial and Intraocular Injections
How to Install a NanoFil Syringe on an UltraMicroPump
How to Setup the NanoFil System for Making Injections
NanoFil Microliter Syringes for Accurate Microinjections
NanoFil Needle Cause Less Tissue Trauma By Design

| Tip Order Number | Tip O.D. (µm) | Tip I.D. (µm) | Tip Length (mm) | Total Length (mm) | Shank O.D. (µm) | Bevel Length (µm) | Tip Material |
| NF33BV | 210 | 115 | 10 | 40 | 460 | ≈348 | Stainless Steel |
| NF34BV | 185 | 85 | 5 | 35 | 460 | ≈290 | Stainless Steel |
| NF35BV | 135 | 55 | 5 | 35 | 460 | ≈204 | Stainless Steel |
| NF36BV | 120 | 35 | 3 | 33 | 460 | ≈156 | Stainless Steel |
| NFQ34-5 | 160 | 100 | 55 | 75 | 460 | Quartz | |
| NF33BL | 210 | 115 | 10 | 40 | 460 | ≈0 | Stainless Steel |
| NF34BL | 185 | 85 | 5 | 35 | 460 | ≈0 | Stainless Steel |
| NF35BL | 135 | 55 | 5 | 35 | 460 | ≈0 | Stainless Steel |
| NF36BL | 120 | 35 | 3 | 33 | 460 | ≈0 | Stainless Steel |
| Silflex | 100 | 35 | |||||
| NF26BV | 460 | 140 | 40 | 460 | Stainless Steel |
NANOFILâ„¢ Syringe Specification Table
| Syringe Order Code | Volume | Syringe Type | Syringe Body | Autoclavable/Gas Sterilizable | Plunger Stroke Length | Plunger Cap Dimensions | Syringe Barrel I.D. / O.D.* | Qty | MICRO4 / MICRO2T Syringe Type Selection |
| NANOFIL | 10µL | Gastight | Borosilicate glass, Stainless Steel, PTFE | Yes ✓ 10°C/50°F to 80°C/176°F, 1000psig max pressure | 60mm | O.D.*: 7.90mm (0.311in) Depth: 2.80mm (0.110in) | 0.46mm (0.018in) / 6.40mm (0.252in) | 1 EA | L (MICRO4) 5 (MICRO2T) |
| NANOFIL-100 | 100µL | 0.46mm (0.018in) / 6.40mm (0.252in) | 1 EA |
*I.D. = Inner diameter, O.D. = Outer diameter


