JoVE
- - July 07, 2014
Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller).
References
Warmerdam, T., Schröder, F., Wit, H., & Albers, F. (n.d.). Perilymphatic and endolymphatic pressures during endolymphatic hydrops. European Archives of Oto-Rhino-Laryngology, 260(1), 9–11. http://doi.org/10.1007/s00405-002-0518-2
Wei, J., Song, J., Jiang, S., Zhang, G., Wheeler, D., Zhang, J., … Liu, R. (2017). Role of intratubular pressure during the ischemic phase in acute kidney injury. American Journal of Physiology - Renal Physiology, 312(6), F1158–F1165. http://doi.org/10.1152/ajprenal.00527.2016
Petrie, R. J., Koo, H., & Yamada, K. M. (2014). Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix. Science, 345(6200), 1062–1065. http://doi.org/10.1126/science.1256965
Petrie,...more - - June 30, 2014
In this JoVE video you can observe as the researchers perform an intraluminal middle cerebral artery occlusion (MCAO) on a mouse. The MCAO model is the most frequent used model among experimental ischemic stroke models.
WPI instruments used in this application include:
SuperCut Iris scissor, straight 14218 Vannas micro-scissor, straight 14003 Dumont fine tip forceps 503290 Vessel clip 14120 High Frequency Desiccator 900 504043 Silicone RTV adhesive 1571 Thermal Cautery Unit 501292 BAT-12 BAT-12R Ansari, S., Azari, H., McConnell, D. J., Afzal, A., Mocco, J. Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice. J. Vis. Exp. (51), e2879, doi:10.3791/2879 (2011).
- - June 24, 2014
Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes:
- - October 02, 2013
WPI surgical instruments were recently featured in a JoVE video that demonstrates a new method for cross pollinating grasses.
Jiang, H., Barbier, H., Brutnell, T. Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses. J. Vis. Exp. (80), e50527, doi:10.3791/50527 (2013).
- - August 22, 2013
Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos. Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.
Check out the JOVE video to see:
- How to make electrodes for electroporation
- How to setup the electroporation
- How to inject the morpholinos
- Examine some of the results
References
JOVE video: Holmes, K. E., Wyatt, M. J., Shen, Y., Thompson, D. A., Barald, K. F. "Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development". J. Vis. Exp. (47), e2466, doi:10.3791/2466 (2011).
Warmerdam, T., Schröder, F., Wit, H., & Albers, F. (n.d.). Perilymphatic and endolymphatic pressures during endolymphatic hydrops. European Archives of Oto-Rhino-Laryngology, 260(1), 9–11. http://doi.org/10.1007/s00405-002-0518-2
Wei,...more
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