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Each NanoFil needle is individually handcrafted and made for versatility to serve your low-volume sample delivery needs. All needles are made with a universal 26G fixture to support and house each gauge of needle we offer: 33, 34, 35, and even 36G in blunt or beveled styles. WPI currently offers the smallest commercially available needles on the market—perfect for extremely sensitive targets, such as in ophthalmic applications. The universal fixture allows all needles to be used interchangeably across any NanoFil syringe.  Any NanoFil needle can be inserted directly into the barrel of any NanoFil syringe, creating a 1:1 connection with the plunger, resulting in virtually zero dead volume. The sealing gasket seating the needle to the syringe reinforces a tight, uniform fit for a truly gas tight environment. Our design compatibility supports your ever-evolving application needs across varying delivery volumes required. 

Are you looking for a microliter or sub-microliter and high precision syringe that holds needles as small as 36 gauge (G), in addition to having the capability to connect to quartz tubing?

WPI’s NanoFil is the answer. We offer NanoFil syringes with NanoFil needles or the option to connect the NanoFil syringe to quartz tubing to use in research studies, mainly involving sub-microliter volume injections into animal tissues.

At WPI, we know precision and ease of use matter, especially when you're handling microinjections at the smallest scales. That’s why we’ve created a quick unboxing and setup guide to help you hit the ground running with your new NanoFil™ gas-tight syringe system.

Whether you're new to NanoFil™ or just want a refresher on what's included and how to get started, this article walks you through everything you need to know.

WPI’s NanoFil™ Gas-Tight syringe offers the ultimate precision for sensitive tissue, particularly for ophthalmic-specific applications. The injection system boasts zero dead volume in the terminal between the interchangeable needle and plunger— creating a truly gas-tight system once primed. NanoFil™ needles are offered in blunt or beveled styles down to 36G, the smallest commercially available gauge on the market.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.

The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the needle or the gasket.

1. To change or install a needle, loosen the screw cap by turning it counterclockwise to release the compression of the seal. If there is a needle in place, it should pull out easily.
   
   
2. Remove the screw cap and the gasket from the syringe.
   
   
3. Install...more

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It comes in both 10 and 100 microliter volumes and can be paired with a UMP3 UltraMicroPump for making accurate microliter injections. NanoFil stainless steel needles range in size from 33 to 36 gauge.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It makes quantitative nanoliter injection much easier and more accurate than any other method.

NANOFIL is a 10microliter syringe, and NANOFIL-100 is a 100 µL syringe. The large volume of the syringe is useful for applications that require multiple injections. The speed of the flow at the needle of the NANOFIL-100 can be 10 times faster than the 10 µL NanoFil when used with UMP3 UltraMicroPump. The limitation of the 100 µL syringe is that the injection volume resolution is lower than that of the 10 µL syringe.

Reduced Needle Size and Dead Volume

The design reduces the...more

NanoFil™ is a unique low volume gas tight syringe developed for improved microinjection in mice and other small animals. Here we show you several ways in which the NanoFil can be used for making injections. You can install the syringe on WPI’s UMP3 UltraMicroPump, using SilFlex tubing and a holder, so you can accurately inject by hand. You can install it in a stereotaxic frame. You can also directly inject by hand.

NanoFil can be used in several different configurations:

1. Install it on WPI’s UMP3 UltraMicroPump, which allows nanoliter resolution and reproducibility. For neural system injection, you can mount the UMP3 on a stereotaxic frame.
   
   
2. You can use SilFlex tubing and a holder. Mount the needle on a small plastic holder and connected it to the NanoFil by a 35 cm length of flexible tubing. Then mount the NanoFil on the UMP3 pump. This lets you hold the animal in one hand and insert the needle with the other. When the needle reaches the desired location, activate the pump using the...more

When working with gas-tight NanoFil™ syringes, especially in applications involving viral vectors or delicate biological samples, priming your syringe correctly is essential to ensure reliable and accurate sample delivery. Even microscopic air pockets can lead to inconsistent dosing or air injection, which can be detrimental in high-precision workflows. Here's a step-by-step guide to priming your NanoFil™ syringe system effectively.

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. NanoFil needles are specially designed with a small outside diameter and spear point for shallow penetration and to cause the least amount of tissue damage. 

Specially designed tips as small as 36 gauge (110 micron OD) are offered in both blunt and beveled styles. These tips will cause less trauma to the tissue than any other form of micro syringe currently in use. NanoFil can be used with many forms of small tubing and tips coupled with the syringe barrel.

• The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed.
• The beveled style is used for applications that involve penetration of tough tissue.

Diffusion at the Tip Minimized

With a 36-gauge needle installed on the NanoFil, the error caused by diffusion at the tip is be reduced to the sub-nanoliter level, compared with the upper 10s of nanoliter range of 100 µm syringes...more

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. During our field trials, the 35-gauge needle was scientists’ most popular and preferred NanoFil tip. The combination of its strength, length, durability and clogging resistance creates a solid balance.

The 33-gauge tip is similar to Hamilton's 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. 33-gauge tips used to be the smallest size sold, but our 35-gauge tip is much better for injections involving small animals, especially mice.

Reduce Penetration Depth by 80%

By choosing the 35-gauge tri-beveled NanoFil tips, you can reduce the depth of penetration by almost 80% when compared with Hamilton's 33-gauge, 10-degree beveled tip. The NanoFil penetration is 230 microns versus 348 for the Hamilton. The smaller tip size significantly...more

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Using a UMP3 UltraMicroPump combined with the syringe allows you to make repeatable injections in the nanoliter range. Here we'll show you how to install a NanoFil Syringe on the UMP3 UltraMicroPump.

NanoFil syringes are 10 µL and 100 µL syringes which can be installed on a UMP3 UltraMicroPump to give you repeatable injections in the nanoliter range. We will use the collar of the NanoFil syringe and the plunger as the two points to anchor the syringe to the pump.

1. The syringe clamps are located at the end of the UMP3 pump head. Depress the clamp release button to open the syringe clamps. 
   
   
2. Position the cap of the plunger into the plunger cap holder at the rear end of the UltraMicroPump.
   
   
3. Then slide the syringe collar into the into position next to the collar stop. 
   
   
4. Slide the syringe body into the syringe clamp so that the syringe collar fits snugly against...more

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we'll show you how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

The NanoFil syringe system utilizes a seal system that is based on a 0.46 mm shank diameter. Any needle or tubing with an outside diameter of 0.46 mm should fit the NanoFil’s seal system. Here is how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

PRO TIP: Always use surgical gloves to prevent the oils from your skin from affecting the needle or the gasket.

1. To change or install a needle, loosen the screw cap by turning it counterclockwise to release the compression of the seal. If there is a needle in place, it should pull out easily.
   
   
2. Remove the screw cap and the gasket from the syringe.
   
   
3. Install the replacement needle by pushing the shank of the needle into the open hole in the screw cap...more

Microinjection uses either a metal needle or a glass micropipette to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic setup may be required.

WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. Here we will highlight our microinjection pumps. The setup you choose depends on the size of injection aliquots, the volume to be injected and the size of needle or glass tip chosen. The products described below are listed in order by the smallest volume they can inject. See the comparison chart below or the related post links at the bottom.

Pressure Injectors

WPI's new generation of pressure microinjectors are quickly replacing the very popular legacy line of pnuematic valve PicoPumps Pressure Injectors. The three new injectors facilitate any type of...more