Page 3 - Epithelial Physiology

Transepithelial/Endothelial Electrical Resistance (TEER) qualitatively measures cellular monolayer health and quantitatively evaluates cellular confluence by measuring the electrical resistance across a cell monolayer. It is commonly used to assess the integrity and permeability of cellular barriers, such as epithelial and endothelial cell layers in cell culture plates. Organ-on-Chip platforms are an emerging market for TEER applications. Let’s look at what TEER is and how it is used.

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on 24-well or 96-well high throughput screening (HTS) plates utilizing our innovative EVOM technology, qualitatively measuring cell monolayer health and quantitatively measuring cellular confluence by reaching an increase or a plateau in tissue resistance. The autosampler has a 3-well rinse station for cleaning and stabilizing the electrodes. Now, sterile disposable rinse station inserts are available for your convenience. They fit over the 3-well base rinse station. You can simply throw out the disposable inserts with the solutions when you are changing plates. This short videos shows how easy they are to use.

The process of drug discovery and development is extremely slow and expensive. Regulatory authorities, such as US FDA need animal models to test absorption, distribution, metabolism, and excretion (ADME) which estimates safety and effectiveness of a drug. 

Transepithelial Electrical Resistance (TEER) measurements play a vital role in assessing barrier function, tissue integrity, and cell-cell interactions in Organ-on-Chip (OoC) platforms. Measuring TEER by applying a small AC current across a monolayer of cells and measuring the resistance to that current, which provides valuable insights into the physiological relevance and functionality of in vitro models, enabling researchers to study organ-specific responses, disease mechanisms, and drug effects while cells are growing in a controlled microenvironment. This article will explore the applications of TEER in OoC set-ups, critical aspects to TEER measurements in microphysiological systems, and challenges and limitations that must be overcome to fully realize the potential of this technology for OoC platforms.

Cell therapies have revolutionized the field of regenerative medicine, offering promising treatments for a variety of medical conditions. Maintaining the safety, efficacy, and consistency of cell-based therapies is paramount for their successful application in clinical settings. Quality control measures play a vital role in evaluating the quality of these therapies, from development to administration. This article explores the current approaches to quality control in cell therapies, including the utilization of functional measurement techniques such as transepithelial electrical resistance (TEER), to ensure the quality and safety of these promising treatments.

Transepithelial electrical resistance (TEER) has emerged as a powerful tool in ophthalmology research and has become a standard method for study of retinal pigment epithelial (RPE) cell biology and the development of these cells as a therapeutic for retinal diseases such as Age-Related Macular Degeneration (AMD). The application of TEER in assessing the barrier function and integrity of RPE cell layers has significantly advanced our understanding of retinal diseases and paved the way for innovative therapeutic interventions including some of the most promising cell therapies to date. This article explores the use of TEER in ophthalmology research, with a focus on studies utilizing RPE cells for both research and clinical applications.

Choosing a dependable lab equipment supplier is crucial for ensuring the quality, reliability, and timely delivery of the equipment you require.  When your research is on the line, you need a company you trust, a supplier with a proven track record and a service team to back you up. Here are a few important considerations when selecting a lab equipment supplier.

Transepithelial/transendothelial electrical resistance (TEER) measurement is one of the most used practices to evaluate cellular health, such as cellular confluence, barrier integrity, or barrier function of cellular monolayers grown onto multiwells. The TEER measurement using WPI’s Epithelial Voltohmmeter (EVOM) is considered the gold standard because of its reliable measurements and numerous literature citations using various cell types. The EVOM™ Manual and EVOM™ Auto along with different choices of electrodes (STX4, STX HTS high throughput screening, EndOhm chambers, and multielectrode array for EVOM™ Auto) allow researchers to perform and analyze cell samples in 6, 12, and 24 removable inserts and 24 and 96 HTS multiwell plate formats. The major challenges researchers may encounter while performing studies to capture TEER measurement include:
·      Unstable read outs
·      Out of range values 
·      Inconsistent measurements among sample replicates or batches.
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EVOM™ Auto is WPI’s latest high throughput screening TEER measurement system with 24 and 96 HTS transwell plate measurement capability, and it offers easy options for switching between different plates. I will show you how to setup it up. The Interface Unit is the controller that establishes communication between the EVOM™ Auto software and the autosampler. 

EVOM™ Auto High Throughput (HTS) Transepithiel Electrical Resistance (TEER) Measurement System with 24 and 96 transwell capability can switch between different 24 and 96 HTS transwell plates by using matching electrode arrays and plate positioner. Here we demonstrate how to install a well plate. 

The EVOM™ Auto High Throughput (HTS) Transepithiel Electrical Resistance (TEER) Measurement System with 24 and 96 transwell capability has a removable electrode array head. Different electrode arrays can be used with the same system to analyze a variety of 24 and 96 transwell HTS plates. 

EVOM™ Auto High Throughput (HTS) Transepithiel Electrical Resistance (TEER) Measurement System with 24 and 96 transwell capability can switch between different 24 and 96 HTS well plates by using matching electrode arrays and plate positioners. Here we demonstrate how to switch to a 24 transwell plate after using a 96 transwell plate. 

The foundation of many research laboratories is the ability to do in vitro studies, which rely on the ability to culture cells in the laboratory. Cell culture technologies have advanced significantly, allowing researchers to successfully culture many types of cell lines.

The EVOM™ family of TEER measurement meters have been cited in over 16,000 peer-reviewed, scientific journal articles. The EVOM™ Manual has been designed to improve workflow efficiency, increase stability, and provide more repeatable measurements than older Trans Epithelial Electrical Resistance (TEER) meters including the Millipore MilliCell® ERS-2, Millipore MilliCell ERS and World Precision Instruments EVOM2, EVOM and EVOMX. Here’s a quick summary of benefits of the EVOM™ Manual versus the MilliCell ERS voltohmmeter.