Page 20 - Product Information
- - April 25, 2013
Profile A Metal Microelectrodes
Platinum Iridium
These electrodes are sold in packages of 10.
Order code Length Insulation
ThicknessShaft
DiameterNominal
Impedance
±20%Tip
DiameterTypical Use PTM23B05 51mm 3µ 0.254mm 0.5MΩ 1-2µ Single and multi unit recording, stimulation, chronic implants Tungsten
...more
Order codeLength Insulation
ThicknessShaft
DiameterNominal
Impedance
±20%Tip
DiameterTypical Use TM31A10 76mm 1µ 0.127 mm 1.0 MΩ 1µ Multi unit and single unit recording and microstimulation TM31A20 76mm 1µ 0.127 mm 2.0 MΩ 1µ Multi unit and single unit recording and microstimulation TM31A50 76mm 1µ 0.127 mm 5.0 MΩ 1µ Multi unit and single unit recording and microstimulation TM31C40 76mm 1µ 0.085 mm 4.0 MΩ 1µ Recording from small tightly packed cells TM33A05 76mm 3µ 0.127 mm 0.5 MΩ 1µ Multi unit and single unit recording and microstimulation TM33A10 76mm 3µ 0.127 mm 1.0 MΩ 1µ Multi unit and single unit recording and microstimulation TM33A20 - - April 25, 2013
Introductory Assortments
Each of these assortment kits includes electrodes with different impedance within each style. Use an assortment kit to determine which electrode you need for your experiment. Each box includes the electrodes listed. There are no mix and match assortment kits.
Profile A
Profile B
Profile C
Assortments Profile A Comparison Chart
Order code Metal Core Contains the following electrode impedances by quantity (pkg of 10) TM31/33Axx Tungsten TM33A05 (2), TM33A10 (3), TM33A20 (3), TM31A50 (2) Assortments Profile B Comparison Chart
Order code Metal Core Contains the following electrode impedances by quantity (pkg of 10) TST33AxKT Tungsten TST33A05KT (3), TST33A10KT (4), TST33A20KT (3) Assortments Profile C Comparison Chart
Order code Metal Core Contains the following electrode impedances by quantity (pkg of 10) TM53AxxKT Tungsten TM53A05KT (3), TM53A10KT (3), TM53A20KT (4) Concentric Electrodes*
...moreOrder code Metal Core Length Impedance Probe Outer
Diameter - - April 25, 2013
Superior microelectrodes for outstanding extracellular recording — tungsten, iridium, platinum-iridium, and Elgiloy®
Types of Metal Electrodes
WPI offers a large variety of metal electrodes and they come in three basic styles (profiles). The selection guide that follows references the three electrode profiles. In addition, concentric bipolar electrodes are also discussed, as well as some of the options offered for the metal electrodes.
NOTE: The electrode diagrams below are not shown to scale.
Profile A
Profile B
Profile C
Concentric Bipolar Electrodes
These electrodes are excellent for shielded macro recording, as well as evoked potentials. They are especially well suited for bipolar stimulation. The tungsten electrode is sharpened to a point and is 75μm in diameter. The outer stainless steel conductor is insulated with Polyimide tubing to within 0.2mm of the end of the stainless steel tube. This electrode is also available...more
- - April 25, 2013
Eliminate the cost and trouble of making your own micropipettes — WPI can quickly supply your need for consistently sized pre-pulled glass micropipettes for injection of dyes or proteins into cells, oocytes and for many other biomedical laboratory applications. Tip diameters (ID) range from 0.1 to 10 micrometers. Micropipettes are available as plain shank or with luer fittings. - Schott Duran borosilicate glass
- 0.5 micrometer and smaller ID micropipettes include an internal glass fiber for easy filling
- Tip inner diameter tolerance ±20%
- Short taper yields high strength
- Nominal length ≈ 50 mm
- OD:ID = 1.33:1
- Standard capillary outer diameters are 1.0 mm (thin-wall) or 1.14 mm
- Every pipette individually tested and inspected
- Vacuum packed
Silanized Tips (Luer Shank)
Silanization waterproofs the glass to retard water when inserting into cell. This will not let the outside fluid run down the pipette and get inside so easily.
...moreOrder code Shank Tip I.D. Shank Length Glass O.D. Filament Fire Polished - - April 25, 2013
The 14011 barbed tubing assortment kit contains over 250 items. All the items are pictured on a grid below. The darker lines in the image grid represent centimeters, and the smaller ones are 2.5mm apart.
- - April 25, 2013
A 4-way stopcock allows for 360 degrees of rotation and has the states (shown below) for each of the four available positions. A 3-way stopcock has only three positions and has the first three states shown below. In the first state, liquid flows between points A and B.
In the second, it flows between points A and C.
In the third, it flows between points B and C.
In the fourth state (4-way only), it flows between all three points.While they are sold in the popular kit 14011, they are also sold separately:
- 14035-10–4-way Stopcock, Luer Lock (pkg. of 10)
- 14036-15–4-way Luer Stopcock (pkg. of 15)
- 14057-10–4-way Stockcok, Luer Lock, Blue (pkg. of 10)
- - April 25, 2013
WPI offers an array of metal microelectrodes. In this guide, we will look at introductory assortments, concentric electrodes, profile A, profile B and profile C electrodes. For basic information on these types of metal electrodes, see the Metal Microelectrode Basics page. Links to these related posts appear below this article.
Eligoy Steel is a *Cobalt/chromium/nickel alloy.
Deciphering Part Numbers
With few exceptions, the WPI metal electrodes conform to a part number standard depicted below. For the part number shown, you can determine that:- Metal - The electrode is made of platinum iridium. (See the metal codes below.)
- Length in inches - It is 2" long.
- Coating Thickness in microns - It has a 3µm coating.
- Core shaft diameter in millimeters - "B" electrodes with Kapton coating are 0.356mm.(See the core shaft diameter description below.) This code doesn't apply to Elgiloy stainless steel electrodes.
- Impedance at 1kHz, measured in 0.1MΩ - This electrode has 1.0MΩ nominal impedance...more
- - April 24, 2013
Luer kits contain many plastic parts made of a variety of materials. A common question is how to sterilize these parts. Common sterilizing methods include:
- EtO (gas)–a chemical steriliant small enough to penetrate the microbial cells that destroying nuclear cell components
- 70/30 Alcohol
- Cidex (Activated Dialdehyde solution)
- Autoclaving (wet 121°C, 15PSI for 30 minutes)–Before autoclaving, verify that the caps are properly loosened or removed to prevent any accidental implosion. Carefully clean the items with distilled water before autoclaving, because some chemicals that are inert on plastic resins at room temperature causes deterioration at high temperatures.
Can the Luer Kits be Autoclaved?
We offer to following luer kits. This table shows the materials used in each kit and whether or not the plastic parts are autoclavable.
Order code Materials Autoclavable 504955 Nylon NO 504954 Polypropylene Yes 14011 Variety Some pieces can be What Materials are Autoclavable?
This table shows a...more
- - April 24, 2013
WPI offers a variety of precious metals and wire. The table below compares the specifications of the various wires. The following hints are offered to help you understand the part number codes and choose the wire you need:
- Micro coaxial cables (MAXxxxx) are ideal for microelectrode fabrication and construction of similar research tools. The dual shielding eliminates electrical interference caused by radio frequencies (RF), electrostatic and microphonics (e.g., bending and vibration). Available with single or dual (twin) conductors.
- Thermocouple wire is for temperature monitoring. TCK3050 has a chromel/alumel pair, for use in inert or oxidizing atmosphere at 1500-1800 °F.
- Teflon-coated stainless steel (type 304) wire (SSTxxxx) is available in 25-ft and 50-ft lengths. The Teflon coating is 4µm thick.
- Carbon wire (C3005) is a single 30µm fiber of electrochemically activated carbon. This fiber is especially useful in micro-electrochemical experiments.
- Carbon filament wire (...more
- - April 24, 2013
WPI’s microelectrode holder-half-cells couple fluid-filled glass micropipettes to high input impedance amplifiers. A Ag/AgCl pellet (or a silver wire) molded into the holder body provides stable potential. Electrical connection is made via male 2mm pins or female 2mm sockets. The pipette may be mounted axially or at right angles to the holder. Pipettes are held with screw-caps or rubber gaskets (without caps). Filling WPI microelectrode holders with electrolytes containing chloride results in stable electrode potential. Suitable electrolytes include KCl, NaCl andCaCl2. Holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0mm outside diameters. (Call WPI regarding custom designs for other glass diameters.) The holder style you select will depend on your experimental application, space, and instrumentation.
Hints for selecting and ordering micropipette holders
- Determine the required electrical connection on the holder. For example, if you wish to connect...more
- - April 24, 2013
Choosing the right parts for your application is critical. Below you will find a table comparing many common parts to help you determine just what you need.
Cables
...moreOrder code Application/Description Connector A Connector B Cable Length 2851
Standard BNC cable BNC (male) BNC (male) 5 ft 2 in 3294
Ground wire for DAM80 probe Clip none 3 ft (0.9 m) 3491
Extension for any 8-pin DIN DIN (male) DIN (female) 5 ft (1.5 m) 3517
DAM50, DAM60, DAM70, shielded (two cables/pkg) Modular phone plug, 4 wire none 3 ft (0.9 m) 3578
Adapter cable for Ag/AgCl pellets 2 mm pin none 5 ft (1.5 m) 5371
Low-noise cable for microelectrode holders 2 mm gold pin 2 mm gold pin 2 ft (0.6 m) 5372
Low-noise cable for microelectrode holders 2 mm gold jack 2 mm gold jack 2 ft (0.6 m) 5373
Low-noise cable for microelectrode holders Combination pin/jack Combination pin/jack 2 ft (0.6 m) 5374
Low-noise cable for microelectrode holders BNC (male) 2 mm gold pin 4 ft (1.2 m) 5375
Low-noise cable for microelectrode holders BNC - - April 23, 2013
In this video, WPI Chemist Nikki Scafa demonstrates how to make a 0.1M solution of CuCl2 for calibrating WPI NO microsensors.
- - April 23, 2013
In this video, WPI Chemist Nikki Scafa demonstrates how to make a PBS buffer solution for use with WPI microsensors.
- - April 23, 2013
- - April 23, 2013
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