Page 5 - Product Information

Cell therapies have emerged as a promising approach for treating various medical conditions, offering the potential for regenerating damaged tissues, restoring normal cellular function, or even acting as cytotoxic cells that target and destroy diseased cells such as cancer cells. As the field of cell-based therapeutics and regenerative medicine continues to advance, ensuring the quality and safety of cell-based therapies is of paramount importance. Quality control measures play a critical role in the manufacturing process of cell therapies, with trans-epithelial electrical resistance (TEER) emerging as a valuable tool for assessing the integrity and functionality of cells used in these therapies. Here, we highlight the use of TEER for quality control of Retinal Pigment Epithelial (RPE) cells, a promising cell therapy for degenerative retinal diseases1.

Cell therapies have revolutionized the field of regenerative medicine, offering promising treatments for a variety of medical conditions. Maintaining the safety, efficacy, and consistency of cell-based therapies is paramount for their successful application in clinical settings. Quality control measures play a vital role in evaluating the quality of these therapies, from development to administration. This article explores the current approaches to quality control in cell therapies, including the utilization of functional measurement techniques such as transepithelial electrical resistance (TEER), to ensure the quality and safety of these promising treatments.

Transepithelial electrical resistance (TEER) has emerged as a powerful tool in ophthalmology research and has become a standard method for study of retinal pigment epithelial (RPE) cell biology and the development of these cells as a therapeutic for retinal diseases such as Age-Related Macular Degeneration (AMD). The application of TEER in assessing the barrier function and integrity of RPE cell layers has significantly advanced our understanding of retinal diseases and paved the way for innovative therapeutic interventions including some of the most promising cell therapies to date. This article explores the use of TEER in ophthalmology research, with a focus on studies utilizing RPE cells for both research and clinical applications.

EVOM™ Auto, with both 24 and 96 HTS multiwell plate capability, is a high throughput screening (HTS) platform offering fast, noninvasive sample scanning by comparing electrical resistance measurements (transepithelial/transendothelial electrical resistance: TEER). TEER measurement experiments are simple to set up and less time consuming than more complex molecular studies. TEER measurement can be used as the primary scanning method to identify any physiological changes that can then be further evaluated by other methods. EVOM™ Auto can capture TEER measurements in 24 and 96 HTS multiwell plates from Corning, Millipore, or MatTek. The sample preparation time in these HTS plates is efficient, allowing for fast, multi-channel pipetting options.

The EVOM™ Auto autosampler ships with a red electrode array lock in place to prevent the electrode array connection port from moving during transit. Here you can see how to easily remove it without damaging the lock or the autosampler.

Below are some popular pullers from WPI, including Sutter pullers. Here we compare them so you can choose a system that is best suited for your application.

Choosing a dependable lab equipment supplier is crucial for ensuring the quality, reliability, and timely delivery of the equipment you require.  When your research is on the line, you need a company you trust, a supplier with a proven track record and a service team to back you up. Here are a few important considerations when selecting a lab equipment supplier.

Let’s take a look at how to set up a Digital Rat/Mouse, Neonatal Rat Stereotaxic frame. In a few steps you will be up and running.

Transepithelial/transendothelial electrical resistance (TEER) measurement is one of the most used practices to evaluate cellular health, such as cellular confluence, barrier integrity, or barrier function of cellular monolayers grown onto multiwells. The TEER measurement using WPI’s Epithelial Voltohmmeter (EVOM) is considered the gold standard because of its reliable measurements and numerous literature citations using various cell types. The EVOM™ Manual and EVOM™ Auto along with different choices of electrodes (STX4, STX HTS high throughput screening, EndOhm chambers, and multielectrode array for EVOM™ Auto) allow researchers to perform and analyze cell samples in 6, 12, and 24 removable inserts and 24 and 96 HTS multiwell plate formats. The major challenges researchers may encounter while performing studies to capture TEER measurement include:
·      Unstable read outs
·      Out of range values 
·      Inconsistent measurements among sample replicates or batches.
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Transepithelial Electrical Resistance (TEER) is a widely used technique in the life sciences fields and therapeutic development. It measures the electrical resistance across a cell monolayer, providing information about the integrity and functionality of epithelial barriers. TEER has proven to be a valuable tool in various fields, including drug absorption studies, tissue engineering, and disease modeling. This article aims to explore the benefits of TEER and its applications in different research areas.

EVOM™ Auto High Throughput (HTS) Transepithiel Electrical Resistance (TEER) Measurement System with 24 and 96 transwell capability can switch between different 24 and 96 HTS well plates by using matching electrode arrays and plate positioners. Here we demonstrate how to switch to a 24 transwell plate after using a 96 transwell plate.