Page 6 - Blog
- - February 02, 2025
Sylgard is a 2-part silicone elastomer with a very low dielectric constant, which makes it ideal for patch clamping and many other lab applications, especially in electrophysiology. The twin pack is a convenient, single use sleeve containing both parts of the adhesive. We will show you how to properly mix the Sylgard Twin Pack.
- - February 01, 2025
WPI's EndOhm chambers are used with WPI's EVOM2 meter to measure TEER (transepithelial electrical resistance) in cell culture inserts. Three chamber sizes are available for 6-well plates, 12-well plates and 24-well plates. Here Subhra demonstrates how to choose an EndOhm chamber to match the cell culture inserts you are using.
- - February 01, 2025
The physical properties of glass depend upon the chemical composition of the glass, as well as how the glass was manufactured. Glass has no set melting point, but four temperatures are of primary importance when discussing glass production.
- - January 20, 2025
EVOM™ Auto is a high throughput screening (HTS) platform offering fast, non-invasive sample scanning by comparing electrical resistance measurements (transepithelial/transendothelial electrical resistance: TEER). TEER measurement experiments are simple to set up and less time consuming than more complex molecular studies. The GxP module offers 21 CFR Part 11 compliant software that is required by the FDA. In this video, we will see how to delete an EVOM™ Auto GxP measurement sequence you no longer need.
- - January 17, 2025
One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your ENDOHM don't stabilize, you may need to do some troubleshooting.
- - January 15, 2025
EndOhm chambers are designed for making accurate TEER measurement of epithelial and endothelial cell cultures. The design keeps the top and bottom electrodes at a fixed gap and maintains the sample (cell culture insert) in a centered position inside. This design minimizes the variability associated with electrode positioning and gap, making EndOhm chambers more accurate and capable of generating reproducible results. WPI made some recent upgrades to the EndOhm chambers.
- - January 15, 2025
Both Kwik-Sil and Kwik-Cast are silicone adhesives, but they are a little different.
- - January 14, 2025
WPI’s EVOM™ line of transepithelial electrical resistance (TEER) measurement systems use silver-silver chloride electrodes for taking measurements. Our newest automated TEER measurement unit, the EVOM™ Auto, is available with an optional GxP module to ensure 21 CFR Part 11 compliance. GxP represents the quality guidelines in place to ensure standards are met. It is used for protocols like Good Laboratory Practice, Good Pharmaceutical Practice, Good Clinical Practice, Good Manufacturing Practice, and Good Research Practice.
Regular maintenance keeps your EVOM™ Auto GxP electrodes performing optimally. Periodically, you will need to re-chloride your electrodes. Just follow the steps shown in this video.
- - January 14, 2025
EVOM™ Auto with GxP powered by TotalLab automates your TEER measurements with the confidence that your data security complies with FDA’s 21 CFR Part 11 regulations.
You may calibrate the electrode array head using the internal reference resistor. Nothing involving the motor movement happens.
- - January 12, 2025
The EndOhm series of chambers, when used with WPI’s EVOM2 resistance meter or the Millicell ERS, are designed to provide reproducible and accurate resistance measurements of endothelial tissue in culture cups. Resistance values obtained with the EndOhm are consistent with those obtained using a well-designed Ussing Chamber.
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