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Tax Exempt Application

World Precision Instruments' (WPI) legacy Robotic Epithelial Measurement System (REMS) and the new advanced, upgraded version of REMS, EVOM™ Auto, are high-throughput, automated transepithelial/transendothelial electrical resistance (TEER) measurement systems. These robotic systems, combined with EVOM™ technology, expedite TEER measurements.

Have you got the new EVOM Auto TEER Measurement System for 96-well HTS plates? This short videos shows how easy it is to setup.

In the process of ‘cell cycle’, cells grow and divide into two genetically identical daughter cells. It is regulated by a complex signaling pathway which keeps cell homeostasis by regulating cell division and DNA duplication1. On the other hand, because cancer cells grow and divide indefinitely out of cell cycle control, anti-mitotic drugs are used to suppress abnormal proliferation of cancer cells2. In particular, Nocodazole is known to be a representative anti-mitotic drug for cancer treatment, and it has the characteristics of disturbing microtubule dynamics during cytoplasmic and nuclear division3,4.

Cytotoxicity refers to the degree of damage to cells.   caused by chemical substances or physical factors. Measuring it through cytotoxicity assay is essential for drug development and biological research. Cells undergo complex signaling pathways that causes various cell death processes such as apoptosis, necrosis, and necroptosis. However, most cytotoxicity assays are measured at the endpoint that makes it difficult to study the dynamic response of cells to drugs.

In this application note, we aimed to examine the performance of a cytotoxicity assay using real-time imaging. Cells treated with various concentrations of Nocodazole, the anti-cancer drug, were stained with fluorescent dye during cell death, then monitored with Celloger® Mini Plus. It was observed through time-lapse imaging that apoptosis increased in a Nocodazole dose-dependent manner, and the degree of apoptosis was quantitatively measured and graphed using the Analysis software provided with the Celloger® Mini Plus.

As white blood cells responsible for immune function are suspension cells that travel along blood vessels, immunology studies often use various suspension cell lines originating from white blood cells. Dealing with suspension cells, unlike adherent cells, slight movement of a plate when locating it on the microscope causes the cells to float. Aside from the problems caused by temperature and CO2 instability, it is in fact not possible to use a traditional microscope to monitor cells in real time. Therefore, in order to stably monitor suspension cells, a live cell imaging device such as Celloger® Mini Plus that operates inside an incubator is essential1. In addition, with Celloger® Mini Plus, the camera inside the system moves to capture the images of cells in multiple positions to keep the cell sample in a steady state instead of having a movable stage with a plate on it. When the suspension cells were monitored both by Celloger® Mini Plus and microscope, imaging with Celloger® Mini Plus...more

The US FDA Modernization Act 2.0 replaces the 1938 Federal Food, Drug and Cosmetic Act which required animal testing prior to human trials. The new law, sponsored in the Senate by Rand Paul and co-sponsored by 11 other bi-partisan senators, eliminates that requirement. Representatives Vern Buchanan and Elaine Luria introduced the companion bill in the House, with over 105 bi-partisan cosponsors. President Biden signed the bill into law in December 2022.

With the advent of modern research methods, we now have many options available to test the efficacy and safety of new pharmaceuticals without requiring the use of animals. Some of these methods include cell-based assays, microphysiological systems like organ-on-a chip (OOC), and bio-printed or computer models. In 2019, the cost of bringing a new drug to market varied greatly from $161 million up to $4.5 Billion[1]. A 2020 study estimated the median cost at a staggering $985.3 million[2]. It can take up to 18 years to bring a new drug to...more