Page 5 - Lab Supplies
- - September 07, 2020
[by Gabe Gonzalez]
The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch, manually or a computer controlled TTL pulse. The PV850 is designed to inject very small quantities of fluids, such as drugs into cells...more
- - September 02, 2020
Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump.
1. What's Included with a PV Pump
In this first video (#1) you can see all the components of the system as they unpack the system.
2. Connecting Tubing on the PV830
In this video (#2) you see how to connect the hard tubing for both the pressure and the vacuum ports on the PV830. The PicoPumps use quick connectors. Push the tubing in to install it, and press the blue collar and pull on the tubing to remove it from the quick connector.
3. Setting Hold Pressure on a PicoPump...more
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- - July 12, 2020
[by Alec Dickson]
Easy to Configure Using the SmartTouch Interface
The UMP3 UltraMicroPump is easy to configure using the SMARTouch interface. It is pre-programmed for popular microsyringes and allows for user-defined syringe parameters, too. This ensures nearly universal compatibility. You can control up to two pumps, either simultaneously or independently, and the optional foot switch means hands-free operation. You can instantly verify actual injection volume on the graphical display. You can even use the touch screen while wearing gloves.
Ensure Reliable Injections of Small Volumes
The extremely fine thread-pitch of the UMP3 drive shaft ensures reliable injections of small volumes. When you are using a 10-microliter syringe, this correlates to 0.58 nanoliters/step and injections are accurate down to 1 nanoliter. “Smooth” mode uses micro-steps and allows for a smoother injection, increasing the resolution eight-fold. “Standard” mode allows for higher torque, for viscous samples or...more
- - May 14, 2020
The UltraMicroPump3 is a versatile microinjection syringe pump designed to support the needs of a busy lab. The UMP3 Microinjector is suitable for delivery of media from nanoliters to milliliters. the UMP3 injecotor’s user interface and system design enables delivery of highly accurate and repeatable injections. The choice for thousands of scientists, it is widely cited in research papers.
Intuitive Touchscreen Display for Easy SetupThe SMARTouch controller has an intuitive touch screen interface and an easy-to-use menu for quick configuration of the syringe size, volumes and flow rates. The total system calibration is designed to eliminate human errors and reduce experimental processing time. Configuration can be saved and subsequently imported as a script via the USB port.
Control 2 Injectors Simultaneously
The SMARTouch controller can independently control two microinjection syringe pumps (microinjectors) simultaneously, providing complete freedom in the experimental procedures...more
- - March 01, 2020
With the first approved human gene therapy trial in 19891 (Rosenberg, et.al.), gene therapy has come a long way in modern medicine and is making inroads in clinics and the market in general.2,3 2017 was an important year for gene therapy when Luxurna, the first human gene therapy drug for an inherited retinal dystrophy, was approved by Food and Drug Administration (USA).4 Now several drugs are undergoing clinical trials. With an estimated $11 billion (USD) market in the next 10 years, both clinical trials and the pharmaceutical industry are expected to benefit immensely from gene therapy.5
With improved use of viral vectors (Adeno-associated virus (AAV), adenovirus, lentivirus, retrovirus, HSV) or non-viral vectors, research and clinical trials on the development of therapeutic genes have witnessed great success. Viral vectors have been employed for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic, ophthalmologic and infectious diseases and...more
- - August 20, 2019
Are you looking for a microliter or sub-microliter and high precision syringe that holds needles as small as 36 gauge (G), in addition to having the capability to connect to quartz tubing?
WPI’s NanoFil is the answer. We offer NanoFil syringes with NanoFil needles or the option to connect the NanoFil syringe to quartz tubing to use in research studies, mainly involving sub-microliter volume injections into animal tissues.
- - October 03, 2015
The type of pump you choose for your fluid handling will largely depend on your laboratory application. In this article, we will compare the popular pump types.
Microinjectors
WPI Microinjectors use carefully regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive “compensation” pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.
Timing, injection pressure and compensation pressure are adjusted independently using the touch screen interface. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch or a computer-controlled TTL pulse.
The WPI Microinjectors are designed to inject very small quantities of fluids, such as drugs, into cells or small...more
- - June 24, 2015Application kits are specially designed for eye research for injecting retinal pigment epithelium (RPE) and for intraocular (IO) injections. In addition, these kits may be used for brain injection in mice. They need to be used with a NanoFil syringe and UMPIII to achieve accurate, repetitive and oil-free injection in the submicroliter range.
Each kit includes two pieces of Silflex tubing (one for a spare), a holder assembly, spare gaskets, and an assortment of four tips - blunt for the RPE kit and beveled tips for the IO kit. Each kit comes with one each of 33, 34, 35 and 36 gauge tips so that first time users can find the best size for their application.
The Silflex tubing is the most critical component of the kit. This 35cm long, flexible tubing has a very precise outer diameter for airtight fitting with the syringe. It also has a small inner diameter for minimum dead volume, and is very durable when handled correctly. The SilFlex is coupled to the injection tip with a mechanism similar...more
- June 11, 2015First, let's consider volumes. - A milliliter (mL) is one thousandth the volume of a liter (L) or 10-3L
- A microliter (µL) is one thousandth the volume of a mL (10-6L)
- A nanoliter (nL) is one thousandth the volume of a µL (10-9L)
- A picoliter (pL) is one thousandth the volume of a nL (10-12L)
This is graphically represented at the right. Notice that the mL is one trillion times larger than the picoliter. The table (right) shows that the side of a cube with a volume of 1mL is 1cm long. Likewise, the side of a cube with a volume of 1pL is 10µm long. Just for comparison, it shows that the diameter of a sphere with a volume of 1mL is 1.24cm, and the volume of a sphere with a volume of 1pL is 12.4µm.
With these volume comparisons in mind, lets consider some of the available options for microinjection pumps.
...moreRANGE PUMPS APPLICATIONS Milliliter Range 10-3L Syringe Pumps
Peristaltic PumpsGeneral pumping applications Microliter 10-6L UMP3 Tissue/organ/body injection
MicroperfusionNanoliter - March 05, 2015World Precision Instruments' PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, microperfusion and microinjection. It offers programmable sequences of up to four steps with complete control over the heating, force, movement and cooling time. This allows graduated cycles for a variety of applications. PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm.
- December 07, 2014A calibrated BLPR2 on the LabTrax24T was used to record the results. This is a plot of both pumps on the #14 tubing at 50 RPM into a 22 gauge needle. The flow is estimated to be 9 ml/min at 50RPM.
- August 04, 2014Micro4™ from World Precision Instruments is an easy-to-use but very capable controller for WPI's Nanoliter Injector and UMP3 UltraMicropPump.
- October 02, 2013See how simple it is to operate the new ATC2000 Animal Temperature Controller. The new adaptive mode takes the guesswork out of temperature control so you can focus on the details of your own experiment.
- May 21, 2013
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