Page 10 - Videos
- - August 04, 2014
Micro4™ from World Precision Instruments is an easy-to-use but very capable controller for WPI's Nanoliter Injector and UMP3 UltraMicropPump.
- - July 20, 2014
WPI's Nanoliter 2010 Microinjection Pump is ideal for many applications, including zebrafish, xenopus oocytes and drosophila. MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display.
- - July 07, 2014
Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller).
References
Warmerdam, T., Schröder, F., Wit, H., & Albers, F. (n.d.). Perilymphatic and endolymphatic pressures during endolymphatic hydrops. European Archives of Oto-Rhino-Laryngology, 260(1), 9–11. http://doi.org/10.1007/s00405-002-0518-2
Wei, J., Song, J., Jiang, S., Zhang, G., Wheeler, D., Zhang, J., … Liu, R. (2017). Role of intratubular pressure during the ischemic phase in acute kidney injury. American Journal of Physiology - Renal Physiology, 312(6), F1158–F1165. http://doi.org/10.1152/ajprenal.00527.2016
Petrie, R. J., Koo, H., & Yamada, K. M. (2014). Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix. Science, 345(6200), 1062–1065. http://doi.org/10.1126/science.1256965
Petrie,...more - - July 01, 2014
Watch as Barney and Kelly Boyce set up an InVivo microscope chamber. Ideal for live cell imaging, the chambers, along with heaters, carbon dioxide and oxygen controllers and stagetop environments are sold by World Precision Instruments.
- - June 30, 2014
In this JoVE video you can observe as the researchers perform an intraluminal middle cerebral artery occlusion (MCAO) on a mouse. The MCAO model is the most frequent used model among experimental ischemic stroke models.
WPI instruments used in this application include:
SuperCut Iris scissor, straight 14218 Vannas micro-scissor, straight 14003 Dumont fine tip forceps 503290 Vessel clip 14120 High Frequency Desiccator 900 504043 Silicone RTV adhesive 1571 Thermal Cautery Unit 501292 BAT-12 BAT-12R Ansari, S., Azari, H., McConnell, D. J., Afzal, A., Mocco, J. Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice. J. Vis. Exp. (51), e2879, doi:10.3791/2879 (2011).
- - June 24, 2014
Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes:
- - April 30, 2014
The 900A is designed to measure hydrostatic pressure in small vessels and cells. Pressure ranges of -200 to +400 millimeters of mercury can be measured with stability and accuracy. The system's sensing element is an electrolyte-filled glass microelectrode with a tip diameter range of 2 to 5 microns. Pressures of electrolyte solutions are measured by maintaining a salt concentration gradient at the tip of the sensing electrode in dynamic equilibrium by applying an equal air pressure inside the microelectrode. The pressure reading appears on the front panel display and via the BNC recorder output. Because the piezo electric pressure controller uses external pressure and vacuum sources, pressures lower than -200 to greater than +400 mm Hg can be quickly and accurately measured at the microelectrode tip.
Now, you can see how to setup your 900A Micropressure system.
Watch how to regulate the pressure and the vacuum for your 900A using a PM015 pressure manometer.
If you...more
- - October 02, 2013
See how simple it is to operate the new ATC2000 Animal Temperature Controller. The new adaptive mode takes the guesswork out of temperature control so you can focus on the details of your own experiment.
- - October 02, 2013
WPI surgical instruments were recently featured in a JoVE video that demonstrates a new method for cross pollinating grasses.
Jiang, H., Barbier, H., Brutnell, T. Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses. J. Vis. Exp. (80), e50527, doi:10.3791/50527 (2013).
- - September 15, 2013
- - September 15, 2013
The PSMB5N Surgical Microscope has a motorized focusing system that allows for hands free operation. It is lightweight, compact and easy to maneuver. Dual bulbs prevent illumination failure during surgery. It has an optional video adapter and five magnification steps. This video shows you how to assemble your microscope.
Safety is a primary concern when setting up equipment. Here are a few pointers.
- This setup requires open space to work in.
- Be sure to remove the packing materials as soon as you unpack your boxes.
- Watch your hands when you are using a box cutter.
- The articulating arm is spring loaded. Be sure to release the tension on it in a controlled manner.
- Be sure to remove all parts from the boxes before you throw the boxes out.
- The base of the microscope is heavy. It requries two people to remove the base from the box. If you drop the base, you will likely break the casters.
- Do not invert the microscope head with the eyepieces in place. They could fall out and are expensive to...more
- - August 22, 2013
Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos. Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.
Check out the JOVE video to see:
- How to make electrodes for electroporation
- How to setup the electroporation
- How to inject the morpholinos
- Examine some of the results
References
JOVE video: Holmes, K. E., Wyatt, M. J., Shen, Y., Thompson, D. A., Barald, K. F. "Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development". J. Vis. Exp. (47), e2466, doi:10.3791/2466 (2011).
Warmerdam, T., Schröder, F., Wit, H., & Albers, F. (n.d.). Perilymphatic and endolymphatic pressures during endolymphatic hydrops. European Archives of Oto-Rhino-Laryngology, 260(1), 9–11. http://doi.org/10.1007/s00405-002-0518-2
Wei,...more
- - May 21, 2013
- - April 23, 2013
In this video, WPI Chemist Nikki Scafa demonstrates how to make a 0.1M solution of CuCl2 for calibrating WPI NO microsensors.
- - April 23, 2013
In this video, WPI Chemist Nikki Scafa demonstrates how to make a PBS buffer solution for use with WPI microsensors.
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