Page 6 - Lab Supplies
- - April 30, 2013
Surgical Loupes help to alleviate eye strain by enlarging the image when you are working on tiny subjects or conducting precision operations. They are portable and easier to use than a surgical microscope. However, they are not created equal, and choosing the pair that's right for you is important to your satisfaction.
Factors Involved in Choosing Loupes
Choosing the correct surgical loupes for your application involves several factors, including resolution, working distance, field of view, depth of field, magnific
ation , weight and interpupillary distance. These terms are defined below.Ideally, you want the lowest magnification that is suitable for your application. As a general rule, the lower the magnification, the greater the depth of field and field of vision. Likewise, the longer the working distance, the greater the field of view. The larger your field of view, the less you need to turn your head. This reduces eye strain and fatigue. It is also important...more
- - April 30, 2013
The barbed tubing assortment kit contains over 250 items. All the items are pictured on a grid below. The darker lines in the image grid represent centimeters, and the smaller ones are 2.5 mm apart.
...moreLuer to Tubing Coupler Assortment Kit P/N 504955 (Nylon)
1/16" Barbed Male Luer w/ Locking Nut, Qty: 10 3/32" Barbed Male Luer w/ Locking Nut, Qty: 10 1/8" Barbed Male Luer w/ Locking Nut, Qty: 10 1/16" Barbed Male Slip Luer, Qty: 10 3/32" Barbed Male Slip Luer, Qty: 10 1/8" Barbed Male Slip Luer, Qty: 10 1/16" Female Luer for Bulkhead Mounting 1/4" - 28, Qty: 10 3/32" Female Luer for Bulkhead Mounting 1/4" - 28, Qty: 10 1/8" Female Luer for Bulkhead Mounting 1/4" - 28, Qty: 10 Female Luer Connector w/ Full Luer Threads, Qty: 10 Female Luer Tee w/ Full Luer Threads, Qty: 10 10-32 UNF Threaded Port Male Slip Luer, Qty: 10 1/4-28 UNF Threaded Port Male Slip Luer, Qty: 10 10-32 UNF Threaded Port Female Luer, Qty: 10 1/4-28 UNF Threaded Port Female Luer, Qty: 10 1/16" Barbed Female Luer - - April 30, 2013
The Nanoliter injector is a micro-processor controlled injection system that uses direct piston displacement. The collet assembly (exploded view) is shown in the image below. The glass pipette fits into the white, plastic sleeve (spacer). When the collet is screwed in place securely, the black rubber washers are compressed and hold the glass firmly in place.
Nanoliter 2000
A side view of the assembled Nanoliter injector is shown below.
Nanoliter 2010
As the plunger is depressed, it pushes into the glass to expel the liquid in the pipette. Eventually, the diameter of the plunger matches that of the glass, and the plunger cannot expel any more liquid. If the plunger continues to press on the tip of the pipette, the pipette could crack or become dislodged from the plastic sleeve (spacer) inside the collet assembly. If that happens, the Nanoliter will begin to leak.
- - April 30, 2013
...moreOrder code Description DMF1000 Description: Complete Microforge System; includes Programmable Digital Controller and Microscope Application: Fabrication of special shapes of glass micropipettes. For example, pressure polishing of patch clamp pipettes and making of holding pipettes. Unique for pipette tip calibration and microinjection pipettes. Features: Most sophisticated and only microforge on the market with built-in pressure polishing capability. Programmable controller with 10 user-selectable memory storage (of heat and time) for reproducibility. MF200 Description: Complete Microforge System, with analog controller Application:Use to fire polish glass micropipettes and prepare special shapes Features: Comes with W30S Microscope, a 40x objective and three filament sizes. Uses exclusive Kohler illuminator instead of industry standard frosted glass illuminator for less glare and sharper image. OMEGA-Z Description: Omega-Tip-Z with Probe and Holder Application: Measures impedance - - April 29, 2013
Use the table below to compare WPI and Bovie part numbers.
...moreOrder code Bovie P/N Description 500384 AB03 Micro Bur Handle, 1225 rpm 500385 AB04 Micro Burr Handle, 1225 rpm 500386 AB01 Burr Tip, 1Mm Dia., 10/Box 500387 AB05 Burr Tip, 0.5Mm Dia., 10/Box 500388 AA11 Cautery, Adj. High Temp, 10/Bx 500389 HIT1 Cautery, High Temp, 2200ºF 500390 HIT0 Cautery, Low Temp 500391 DEL0 Cautery, Low Temp Kit 500392 DEL1 Cautery, High Temp Kit 500393 H103 Loop Tips, Disposable Hi Temp 500394 H101 Fine Tips, Disposable Hi Temp 500395 H104 Elongated Tips, Low Temp, 500396 H100 Fine Tips, Disposable Low Temp 500421 A1250 Electrosurgical Generator 501001 A1252C Connecting Cord, Reusable 501005 A820 Bipolar Forcep, Bayonet 7.5" 501009 A824 Forceps,Bipolar 4.5", Cvd,Fine 501010 A825 Forceps, Bipolar, 5" Str Med 501011 A826 Forceps,Bipolar, 4.5" Str Fine 501018 A835 Electrode,Ball,Long 501019 A836 Electrode,Needle,Long,Angled 501037 Ball Electrode, 3/16, 25/Box 501056 ESP4 Pencil, Smoke Evacuation 501068 ESPR - - April 29, 2013
- Flexible Teflon microprobes are used for implantation in tissue, in spectrophotometer cuvettes, rectally in neonatal mice, in water baths, PCR thermal cyclers, etc.
- Animal rectal temperatures during surgical procedures and pyrogen testing.
- Skin temperature measurement during exercise physiology studies.
When precise temperature measurements are required, WPI can provide you with a very accurate monitor and thermocouple microprobes. WPI monitors have both resolution and accuracy of 0.1°C in the 0-50°C range and are traceable to NIST standards, whereas, other competitive electronic thermometers have an accuracy that is usually to 0.5°C or worse. Furthermore, all our type T clinical probes are guaranteed accurate to 0.1°C, due to our stringent wire standards. These are five times more accurate than competitive probes made with regular “Special Limits” wire.
Probe Sterilization Methods:
The needle microprobes and animal rectal probes are all designed to withstand sterilization by ETO gas...more
- - April 28, 2013
These instructions describe how to properly focus a binocular microscope.
- Turn on the lamp and adjust its intensity.
- Adjust the interpupilary distance so that the two circles of light merge into one.
- Place a sample on the stage.
- Select the 10X objective and look through the right eyepiece only.
- Use the coarse and fine focus control knobs to adjust the focus of the sample.
- When the sample is clearly visible, use only your left eye. Do NOT adjust the focus knobs. Instead, adjust the diopter on the left eyepiece until the sample comes clearly into view. The image should be clear with both eyes open.
- Choose the desired objective. You may need to adjust the fine focus a little.
- - April 28, 2013
NOTE: For an introduction to microscopes, see Microscope Basics.
A variety of microscope objectives are available. All objectives use lenses to focus light. Light is broken down into various wavelengths (colors) as it travels through a lens. The various wavelengths have different focal points. That means that red, green and blue appears to focus at different points. This is called chromatic aberration. Spherical aberrations are focal mismatches caused by the shape of the lens. Quality lenses are designed correct for chromatic and spherical aberration to bring the primary colors to a common focal point. These terms may help you determine the best objective for your application:
Achromatic objectives–This objective brings red and blue light to a common focus, and is corrected for spherical aberrations for green. It is excellent for black and white viewing. If an objective is not labeled, it is achromatic.
Fluorite or semi-apochromat objectives–These lenses are chromatically corrected...more
- - April 28, 2013
Microscopes are a standard laboratory tool, but purchasing the right microscope for a particular application can be a challenge. First, consider how you will use the instrument. Are you looking at slides, dissecting a small animal or performing a surgery? (The application dictates the necessary working distance and power of magnification.) What kind of a stand will you be using? (Boom stand, articulating arm or post stand) Will the microscope be used in a classroom setting? (A trinocular scope offers the option of including a camera.) Will you need a camera? (A camera allows you to project the microscope image on a PC or TV or to take still images.) The answers to these questions help you determine the required working distance, level of magnification, type of mounting stand and hardware required.
Parts of a Microscope
Eyepiece–Two or more lenses are contained in the eyepiece, and a variety of eyepieces can be used in a microscope. Typical eyepiece magnification ranges are 10X, 15X...more - - April 28, 2013
When it comes to setting up microinjection systems, the options appear endless. The pictures below give some broad suggestions on how you might set up your own system. Keep in mind that many parts are interchangeable depending on your needs or preferences.
In general, you will need a stereo microscope on a stand, a light source, one or two micromanipulators with stands, and one or two injection systems. The following images show various setups for microinjection, and all the WPI part numbers are included for easy reference.
Remember, when you set up your own system, choose the parts that fit your needs. For example:
- M10 or the M9 magnetic base could be used.
- PZMIV stereo microscope could be used instead of the PZMIII stereomicroscope.
- M3301 or the KITE micromanipulators can be used, and these micromanipulators can be placed on either side. (Keep in mind, though, if you wanted to use a KITE micromanipulator on the right side of the setup below, you would order a KITE-R (right hand), or...more
- - April 25, 2013
A 4-way stopcock allows for 360 degrees of rotation and has the states (shown below) for each of the four available positions. A 3-way stopcock has only three positions and has the first three states shown below. In the first state, liquid flows between points A and B.
In the second, it flows between points A and C.
In the third, it flows between points B and C.
In the fourth state (4-way only), it flows between all three points.While they are sold in the popular kit 14011, they are also sold separately:
- 14035-10–4-way Stopcock, Luer Lock (pkg. of 10)
- 14036-15–4-way Luer Stopcock (pkg. of 15)
- 14057-10–4-way Stockcok, Luer Lock, Blue (pkg. of 10)
- - April 25, 2013
The 14011 barbed tubing assortment kit contains over 250 items. All the items are pictured on a grid below. The darker lines in the image grid represent centimeters, and the smaller ones are 2.5mm apart.
- - April 25, 2013
Eliminate the cost and trouble of making your own micropipettes — WPI can quickly supply your need for consistently sized pre-pulled glass micropipettes for injection of dyes or proteins into cells, oocytes and for many other biomedical laboratory applications. Tip diameters (ID) range from 0.1 to 10 micrometers. Micropipettes are available as plain shank or with luer fittings. - Schott Duran borosilicate glass
- 0.5 micrometer and smaller ID micropipettes include an internal glass fiber for easy filling
- Tip inner diameter tolerance ±20%
- Short taper yields high strength
- Nominal length ≈ 50 mm
- OD:ID = 1.33:1
- Standard capillary outer diameters are 1.0 mm (thin-wall) or 1.14 mm
- Every pipette individually tested and inspected
- Vacuum packed
Silanized Tips (Luer Shank)
Silanization waterproofs the glass to retard water when inserting into cell. This will not let the outside fluid run down the pipette and get inside so easily.
...moreOrder code Shank Tip I.D. Shank Length Glass O.D. Filament Fire Polished - - April 24, 2013
Luer kits contain many plastic parts made of a variety of materials. A common question is how to sterilize these parts. Common sterilizing methods include:
- EtO (gas)–a chemical steriliant small enough to penetrate the microbial cells that destroying nuclear cell components
- 70/30 Alcohol
- Cidex (Activated Dialdehyde solution)
- Autoclaving (wet 121°C, 15PSI for 30 minutes)–Before autoclaving, verify that the caps are properly loosened or removed to prevent any accidental implosion. Carefully clean the items with distilled water before autoclaving, because some chemicals that are inert on plastic resins at room temperature causes deterioration at high temperatures.
Can the Luer Kits be Autoclaved?
We offer to following luer kits. This table shows the materials used in each kit and whether or not the plastic parts are autoclavable.
Order code Materials Autoclavable 504955 Nylon NO 504954 Polypropylene Yes 14011 Variety Some pieces can be What Materials are Autoclavable?
This table shows a...more
- - April 24, 2013
WPI offers a variety of precious metals and wire. The table below compares the specifications of the various wires. The following hints are offered to help you understand the part number codes and choose the wire you need:
- Micro coaxial cables (MAXxxxx) are ideal for microelectrode fabrication and construction of similar research tools. The dual shielding eliminates electrical interference caused by radio frequencies (RF), electrostatic and microphonics (e.g., bending and vibration). Available with single or dual (twin) conductors.
- Thermocouple wire is for temperature monitoring. TCK3050 has a chromel/alumel pair, for use in inert or oxidizing atmosphere at 1500-1800 °F.
- Teflon-coated stainless steel (type 304) wire (SSTxxxx) is available in 25-ft and 50-ft lengths. The Teflon coating is 4µm thick.
- Carbon wire (C3005) is a single 30µm fiber of electrochemically activated carbon. This fiber is especially useful in micro-electrochemical experiments.
- Carbon filament wire (...more
